overexpression相关论文
Receptor-interacting Protein 140 Overexpression Promotes Neuro-2a Neuronal Differentiation by ERK1/2
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Up-regulated manganese superoxide dismutase expression increases apoptosis resistance in human esoph
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目的探讨肌动蛋白样6A(ACTL6A)在胰腺癌(PAAD)中的表达、临床意义及其对胰腺癌细胞系SW1990细胞的增殖、凋亡、侵袭和迁移的影响。......
Overexpression of BnKCS1-1, BnKCS1-2, and BnCER1-2 promotes cuticular wax production and increases d
Higher amounts of cuticular wax in plants have been associated with improved plant stress tolerance and increased potent......
Overexpression of tyrosine phosphorylated proteins in reproductive tissues of polycystic ovary syndr
Objective: To identify the alteration of tyrosine phosphorylated protein expression in rats with polycystic ovary syndro......
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
目的探讨超表达Notch胞内结构域(Notch intracellular domain,NICD)对人牙周膜干细胞(human periodontal ligament stem cells,hPDLSC)增......
Myo-inositol-1-phosphate synthase(MIPS) is a key rate limiting enzyme in the de novo biosynthesis of myo-inositol in pla......
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Up-regulated manganese superoxide dismutase expression increases apoptosis resistance in human esoph
Background Esophageal cancer is one of the most common malignancies in the world.In order to identify the proteins assoc......
Overexpression or knock-down of runt-related transcription factor 1 affects BCR-ABL-induced prolifer
Background Runt-related transcription factor 1 (Runx1) plays a crucial role in hematogenesis and its dysfunction may con......
目的 构建鼠疫耶尔森菌中长度在100 nt以内的小RNA缺失株及过表达菌株的方法.方法 在高拷贝质粒pBAD/HisA的基础上利用定点突变试......
Overexpression of microRNA-124 promotes the neuronal differentiation of bone marrow-derived mesenchy
microRNAs (miRNAs) play an important regulatory role in the self-renewal and differentiation of stem cells. In this stud......
Polycomb group (PcG)蛋白家族是一组在胚胎发育中起作用的基因调控因子,根据其功能不同分为polycomb repressive complex l(PRC1)......
Glutamine synthetase (GS, E.C.6.3.1.2) is a key enzyme involved in the assimilation of inorganic nitrogen in higher plan......
High efficient expression of Lhcb2 gene from pea in E. coli and reconstitution of its expressed prod
Lhcb2 gene from pea (Pisum sativum L.) was subcloned into bacterial expression vector pET-3d, and its protein overexpres......
WUSCHEL(WUS)是近年报道的一个重要的干细胞调控基因.本实验用RT-PCR技术从拟南芥(Arabidopsisthaliana L.)中克隆到其cDNA并构建......
Overexpression of a Chloroplast-located Peroxiredoxin Q Gene, SsPrxQ, Increases the Salt and Low-tem
Abiotic stress, such as salt, drought and extreme temperature,can result in enhanced production of reactive oxygen spec......
Overexpression or knock-down of runt-related transcription factor 1 affects BCR-ABL-induced prolifer
Background Runt-related transcription factor 1 (Runx1) plays a crucial role in hematogenesis and its dysfunction may con......
Functional Characterization of Mi, a Root-knot Nematode Resistance Gene from Tomato( Lycopersicon es
Root-knot nematodes (Meloidogyne spp.) cause major economic damage to numerous crop species around the world. Plant resi......
[目的]进一步研究LWT1基因在调控低温和干旱应答中的功能,构建拟南芥LWT1基因过表达载体,获得相应的转基因株系.[方法]以野生型拟......
To construct overexpression lentiviral-based vector carrying rat B7x gene,B7x gene precursor sequences amplified by poly......
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Overexpression of the maize GRF10, an endogenous truncated growth-regulating factor protein, leads t
It has long been thought that growth‐regulating factors (GRFs) gene family members act as transcriptional activators to......
目的探讨慢病毒介导的NLRP3过表达对非小细胞肺癌(NSCLC)A549细胞和NCI-H460细胞的增殖、迁移、侵袭和凋亡的影响,并比较两种细胞......
干旱胁迫严重影响植物的生长发育甚至生存状态,是限制我国西北荒漠植被恢复的主要非生物胁迫因素之一。实验室前期研究发现随黄土......
NtCycB2参与调控烟草腺毛的发生与发育过程,并对多种非生物胁迫具有应答反应。为阐明NtCycB2表达对烟株耐旱性的影响,以敲除NtCycB......
将棉花Steroid 5α—reductase基因(DET2)超量表达载体导入毛白杨中,观察其对毛白杨生长和芽休眠的影响。结果表明,超量表达DET2基因的......
构建番茄SlWRKY53的过量表达载体,通过农杆菌介导转入番茄(Solanumlycoper—sicum)品种AilsaCraig(AC+),获得转基因阳性植株.定量分析显示,......
利用改造后的腺病毒表达载体pDC312-cmv,构建含有8个miR-122表达框的重组腺病毒载体p-8miR-122,通过RT-PCR、ELISA、Sourthern blo......
为调查大肠杆菌高产辅酶Q(CoQ)的可能性,首先研究大肠杆菌生物合成关键基因ubiCA强化表达对大肠杆菌CoQ产量的影响。本文构建了含有ub......
[目的]测定粟酒裂殖酵母高表达的SpTrz2p对其细胞周期的影响。[方法]将粟酒裂殖酵母的trz^2+克隆到pREP4x质粒上,构建带有SpTrz2的......
摘要Kar2p是分子伴侣蛋白,可与新生肽结合,促进新生肽进入内质网。为研究其对米黑根毛霉脂肪酶 (RML) 在毕赤酵母中表达的影响,通过PCR......
利用同源克隆法,克隆了小拟南芥的CBF1基因的cDNA(FJ491244),比对分析结果表明,ApCBF1的cDNA序列的A252,在拟南芥的AtCBF1的cDNA是T2......
目的:研究整合蛋白β1亚基过表达对肝癌细胞SMMC-7721增殖的影响及机制。方法:采用MTT及细胞流式测定观察整合蛋白β1亚基过表达对肝......
已证实乳腺癌细胞表达有VEGF165的第3个受体NRP1,但目前在乳腺癌细胞中过表达VEGF165对肿瘤周围血管生成模式的影响尚未有人研究.......
大豆胞囊线虫(Soybean cyst nematode,SCN)是大豆生产上一种危害严重的世界性害虫,给大豆的产量和品质造成极大的损失。大豆抗性品种......
乙酰乳酸合成酶(ALS)是支链氨基酸、缬氨酸、亮氨酸和异亮氨酸生物合成途径中的关键酶,也是多种除草剂的靶点。为了研究als基因不......
目的:探讨 E2F -1过表达对胃癌 SGC -7901细胞增殖、生长和细胞周期的影响及其分子机制。方法采用携带 E2F -1基因的重组慢病毒颗粒......
目的探讨过表达CAMP反应元件结合蛋白(CREB)在内皮细胞氧化损伤中的作用。方法建立H2O2诱导的血管内皮细胞损伤模型,将内皮细胞ECV30......
FoxM1是Forkhead Box( Fox)转录因子家族的成员之一。近年来研究发现,FoxM1在脑胶质瘤的发生和发展中起着重要的作用。研究表明,人脑胶......
木质部半胱氨酸蛋白酶XCP2(xylem cysteine proteases2)是植物管状细胞凋亡过程中的自溶酶,参与植物与多种病原物互作,但目前未见对......
【目的】磷酸盐转运体运输协助因子(PHF1)通过转录后调节特定磷转运蛋白,影响磷酸盐的利用效率。本研究通过培育过表达OsPHF1的无......
【目的】通过在水稻中过量表达水稻黑条矮缩病毒(Rice black streaked dwarf virus,RBSDV)P7-1基因,分析RBSDV P7-1是否是导致水稻不......
本研究旨在创制OsBTF3过表达转基因水稻株系,为验证OsBTF3基因在水稻抗性和生长发育中的功能、评价其在水稻农艺性状遗传改良中的......
目的:通过同源盒(HOX)B13、C13基因的反义RNA片段的导入,研究子宫内膜癌细胞浸润能力发生的变化。方法:通过质粒转染技术将反义HOXB13......
根据已发表的豆豉纤溶酶基因DNA序列(GeneBank AY720895.2),设计并合成了一对引物,应用PCR技术以筛选的来自豆豉的产纤溶酶的芽孢杆......
目的:构建白念珠菌RAS1基因高表达菌株pCaEXP-RAS1-CAI4。方法:将白念珠菌RAS1基因的开放阅读框(ORF)置于高表达载体pCaEXP的启动子MET......
目的:研究体内S100A6基因过表达对肺腺癌移植瘤生长的影响及其可能机制。方法:18只健康Balb/c雄性裸鼠随机分为三组,空载体对照组、......
旨在克隆从江香猪磷酸化酶激酶γ2(phosphorylase kinase gamma 2,PHKG2)基因编码区,并对PHKG2基因功能进行初步探究。本研究利用RT-......